Validation of a multiplex PCR assay for the simultaneous detection of human papillomavirus and Chlamydia trachomatis in cervical PreservCyt samples.
نویسندگان
چکیده
منابع مشابه
Development of TaqMan Duplex Real-time PCR for Simultaneous Detection of Chlamydia Trachomatis and Mycoplasma Genitalium
Background and Objective: Sexually infections transmitted by bacteria are one of thetherapeutic and social problemsworldwide. The Real-time PCR assay is one of the most sensitive diagnostic and screening methods for these infections. The purpose of this study wassimultaneous detection of Chlamydia trachomatis and Mycoplasma genitaliumusing the TaqMan duplex real-time polymerase chain reaction. ...
متن کاملChlamydia trachomatis detection in cervical PreservCyt specimens from an Irish urban female population.
OBJECTIVE The aim of this study was to determine the prevalence of cervical Chlamydia trachomatis infection by polymerase chain reaction (PCR) in urban women undergoing routine cervical cytological screening and to investigate the relationship with age, cytology, smoking status and concurrent human papillomavirus (HPV) infection. METHODS A total of 996 women (age range 16-69 years) attending ...
متن کاملCorrection: Sensitive Simultaneous Detection of Seven Sexually Transmitted Agents in Semen by Multiplex-PCR and of HPV by Single PCR
Sexually transmitted diseases (STDs) may impair sperm parameters and functions thereby promoting male infertility. To date limited molecular studies were conducted to evaluate the frequency and type of such infections in semen Thus, we aimed at conceiving and validating a multiplex PCR (M-PCR) assay for the simultaneous detection of the following STD pathogens in semen: Chlamydia trachomatis, N...
متن کاملشناسایی مولکولی پاپیلوما ویروس انسانی در نمونه های بافتی بیماران مبتلا به سرطان دهانه رحم به روش PCR چندگانه
Introduction: Contamination with high-risk human papillomavirus (HPV) is one of the most important risk factors for developing cervix cancer. Since there is no possibility of detecting the virus and its subtypes by using current biochemical and serological methods and cell culture, the molecular methods such as multiplex PCR have particular importance in accurate, early and definite diagnosis o...
متن کاملValidation of a Multiplex PCR Assay for the Simultaneous Detection of Human Papillomavirus and Chlamydia trachomatis in Cervical PreservCyt Samples
(MALDI)-TOF MS, with its higher resolution, is the capability of SELDI to enrich low-abundance proteins from complex matrices such as plasma, through, for example, the coupling of specific antibodies to the chip surfaces (4 ). Such an approach is an important methodologic aspect in “second phase” proteomics, which are characterized by repetitive investigation of the same protein to validate the...
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ورودعنوان ژورنال:
- Clinical chemistry
دوره 51 7 شماره
صفحات -
تاریخ انتشار 2005